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CD34+ hematopoietic stem and progenitor cells (HSPCs) play a pivotal role in maintaining and replenishing the hematopoietic system and keep sizeable potential with the treatment method of genetic blood Ailments and immunological health conditions. The emergence of gene modifying technologies, specifically CRISPR-Cas9, has transformed a chance to introduce specific modifications in these cells.
Contact us to debate personalized assay answers for several different other cell sorts to suit your unique needs.
It is important to quench the dissociation reagent utilizing FACS buffer or end medium, by incorporating no less than precisely the same or double the amount on the dissociation reagent.
This solution is suitable for use in the next investigation region(s) as A part of the highlighted workflow stage(s). Discover these workflows To find out more regarding the other items we offer to guidance Each and every exploration region. Exploration Region Workflow Phases
The readings can even be carried out before the culture is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
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TEER measurements is usually performed frequently, without producing damage to the cell culture. You may conduct a weekly TEER time class to describe the barrier purpose in the course of the entire process of ALI society differentiation.
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Certainly, you’ll locate the stage-by-stage protocol for TEER measurement to evaluate the epithelial barrier integrity in ALI cultures below.
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Help you save time by starting off your experiments that has a hugely characterized population of mesenchymal progenitor intermediates